Yes, it does. I have 2.4.4/catalina running on Win2K
The question I think you are meaning to ask is, does jboss run on windows2000, because I am quite sure JBOSS does not in anyway support windows 2000, bill gates has that job covered.
To answer your question.. jboss is coded in 100% java, and java runs everywhere that the JVM has been ported to (which is pretty much everywhere.. including win32 systems)
So yes... if course jboss runs on windows2000...
maybe try a google search before posting such remedial questions to this forum.